Antibacterial activities of the essential oil and hydroethanolic extract from Aeollanthus heliotropioides Oliv

Antibacterial activities of the essential oil and hydroethanolic extract from Aeollanthus heliotropioides Oliv

Authors

  • François Nguimatsia Université des Montagnes, BP 208, Bangangté, Cameroon
  • Sidonie Beatrice Kenmogne Laboratory of Chemistry, Department of Chemistry, University of Douala, PO Box 24157, Douala, Cameroon
  • Madeleine Nina Love Ngo-Mback Institute of Fisheries and Aquatic Sciences - Yabassi University of Douala, Cameroon B.P. 24157 Douala-Cameroon
  • Jonas Kouamouo Université des Montagnes, BP 208, Bangangté, Cameroon
  • Léa Larissa Nzenti Tchuitio Université des Montagnes, BP 208, Bangangté, Cameroon
  • Yannick Kevin Melogmo Dongmo Antimicrobial Agents Unit, Laboratory of Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, Faculty of Science, University of Yaounde´ I, PO Box 812, Yaounde´, Cameroon
  • Pierre Michel Jazet Dongmo Laboratory of Biochemistry, Department of Biochemistry, University of Douala, PO Box 24157, Douala, Cameroon

DOI:

https://doi.org/10.13171/mjc02102021555mnlnm

Abstract

To control bacterial infections, we proposed evaluating the antibacterial activities and modes of action of Aeollanthus heliotropioides essential oil and its hydroethanolic extract. Solvent extracts and essential oil were obtained from the aromatic plant's aerial parts by hydroethanolic maceration and hydro-distillation. The analyses of the chemical composition were performed using gas chromatography coupled with mass spectrometry. The microdilution method evaluated the in vitro antibacterial potential of the essential oil and solvent extracts. The Inhibition of biofilms formation was carried out using a colorimetric biofilm microdilution assay with crystal violet as a dye. The effect of extract and essential oil on the release of nucleic acids was performed using a spectrophotometric method. The time-kill kinetic assay was assessed for hydroethanolic extracts and essential oil. The extraction yield was 0.1%, and the major compounds identified in the essential oil were linalool (43.47%) and cis-α-farnesene (42.67%). The phytochemical screening revealed flavonoids, saponins, phenols, triterpenes, catechin tannins, and quinones. Minimum Inhibitory Concentrations (MICs) ranged from 2.08 mg/mL to 10 mg/mL. Concerning the modes of action, the essential oil showed its bactericidal effect at 2 hours. The reduction of Escherichia coli biofilms formation was found at 0.21 mg/mL. The essential oil treatments resulted in a release of nucleic acids at a concentration of 2.1 mg/mL. These results justify using the essential oil and hydroethanolic extracts of Aeollanthus heliotropioides as a potential source of molecules with antibacterial activity.

References

- L. Cuzin, C. Delpierre, Épidémiologie des maladies infectieuses, EMC - Maladies infectieuses, 2005, 2, 1–5.

- V. R. Racaniello, Emerging infectious diseases, Journal of Clinical Investigation, 2004, 113, 796–798.

- F. Fenollar, O. Mediannikov, Emerging infectious diseases in Africa in the 21st century, New Microbes and New Infections, 2018, 26, S10–S18.

- J. Koyama, Anti-Infective Quinone Derivatives of Recent Patents, Recent Patents on Anti-Infective Drug Discovery, 2006, 1, 113-125. doi:10.2174/157489106775244073.

- R. J. Fair, Y. Tor, Antibiotics and bacterial resistance in the 21st century, Perspectives in Medicinal Chemistry, 2014, 25–64.

- C. De La Fuente-Núñez, V. Korolik, M. Bains, U. Nguyen, E. B. M. Breidenstein, S. Horsman, S. Lewenza, L. Burrows, R. E. W. Hancock, Inhibition of bacterial biofilm formation and swarming motility by a small synthetic cationic peptide, Antimicrobial Agents and Chemotherapy, 2012, 56, 2696–2704.

- I. M. Famuyide, A. O. Aro, F. O. Fasina, J. N. Eloff, L. J. McGaw, Antibacterial and antibiofilm activity of acetone leaf extracts of nine under-investigated south African Eugenia and Syzygium (Myrtaceae) species and their selectivity indices, BMC Complementary and Alternative Medicine, 2019, 19, 1-13. doi:10.1186/s12906-019-2547-z.

- K. Bush, Antimicrobial agents targeting bacterial cell walls and cell membranes, OIE Revue Scientifique et Technique, 2012, 31, 43–56.

- A. S. Nayar, T. J. Dougherty, K. E. Ferguson, B. A. Granger, L. McWilliams, C. Stacey, L. J. Leach, S. Ichiro Narita, H. Tokuda, A. A. Miller, D. G. Brown, S. M. McLeod, Novel antibacterial targets and compounds revealed by a high-throughput cell wall reporter assay, Journal of Bacteriology, 2015, 197, 1726–1734.

- J. H. Rex, B. I. Eisenstein, J. Alder, M. Goldberger, R. Meyer, A. Dane, I. Friedland, C. Knirsch, W. R. Sanhai, J. Tomayko,

C. Lancaster, J. Jackson, Personal View A comprehensive regulatory framework to address the unmet need for new antibacterial treatments, The Lancet Infectious Diseases, 2013, 13, 269–275.

- J. W. Betts, M. Hornsey, R. M. La Ragione, Novel Antibacterials: Alternatives to Traditional Antibiotics, Advances in microbial physiology, 2018, 73, 123-169. doi:10.1016/bs.amps.2018.06.001.

- WHO, WHO Traditional Medicine Strategy 2002–2005, In, Health, World Organization Geneva, 2002, pp, 1–74.

- A. Romulo, E. A. M. Zuhud, J. Rondevaldova, L. Kokoska, Screening of in vitro antimicrobial activity of plants used in traditional Indonesian medicine, Pharmaceutical Biology, 2018, 56, 287–293.

- T. Jiofack, C. Fokunang, N. Guedje, V. Kemeuze, E. Fongnzossie, B. Nkongmeneck, P. M. Mapongmetsem, N. Tsabang, Ethnobotanical uses of medicinal plants of two ethnoecological regions of Cameroon, International Journal of Medicine and Medical Sciences, 2010, 2, 60–79.

- P. M. Mapongmetsem, B. A. Nkongmeneck, Y. V. Pinta, R. Nkuinkeu, N. Tsabang, E. Fongnzossie, V. Kemeuze, T. Jiofack, M. Johnson, S. Asaha, C. Sakwe, C. Mboufack, Etat des lieux des plantes médicinales importantes à conserver et des jardins de plantesmédicinales à promouvoir, In, Rapport CEN/OMS/MEM, , 2007, pp, 1–24.

- M. N. L. Ngo Mback, H. Agnaniet, F. Nguimatsia, P.-M. Jazet Dongmo, J.-B. Hzounda Fokou, I. Bakarnga-via, F. Fekam Boyom, C. Menut, Optimization of antifungal activity of Aeollanthus heliotropioides oliv essential oil and Time Kill Kinetic Assay, Journal de Mycologie Medicale, 2016, 26, 233-243. doi:10.1016/j.mycmed.2016.04.003.

- R. L. Martins, R. C. Simões, É. de M. Rabelo, A. L. F. Farias, A. B. L. Rodrigues, R. da S. Ramos, J. B. Fernandes, L. da S. Santos, S. S. M. da S. de Almeida, Chemical Composition, an Antioxidant, Cytotoxic and Microbiological Activity of the Essential Oil from the Leaves of Aeollanthus suaveolens Mart. ex Spreng, PLOS ONE, 2016, 11, e0166684.

- J. B. Hzounda Fokou, P. M. Jazet Dongmo, I. Bakarnga-Via, M. N. L. Ngo Mback, E. Zeuko'o Memkem, A. Dior. Fall, Emmanuel. Bassene, Fabrice. Fekam Boyom, Optimized combination of Ocimum essential oils Inhibit growth of four Candida albicans, International Journal of Drug Discovery, 2014, 6, 198–206.

- M. N. L. Ngo-Mback, C. Babii, P. M. Jazet Dongmo, M. R. Kouipou Toghueo, M. Stefan, F. Fekam Boyom, Anticandidal and synergistic effect of essential oil fractions from three aromatic plants used in Cameroon, Journal de Mycologie Medicale, 2020, 100940.

- C. Aromdee, P. Wichitchote, N. Jantakun, Spectrophotometric determination of total lactones in Andrographis paniculata Nees. Songklanakarin J. Sci. Technol., 2005, 27, 1227-1231.

- O. O. Odebiyi, E. A. Sofowora, Antimicrobial Alkaloids from a Nigerian Chewing Stick ( Fagara zanthoxyloides ), Journal of Medicinal Plant Research, 1979, 36, 204–207.

- A. Sofowora, E. Ogunbodede, A. Onayade, The role and place of medicinal plants in the strategies for disease prevention., African journal of traditional, complementary, and alternative medicines : AJTCAM / African Networks on Ethnomedicines, 2013, 10, 210–229.

- W. Evans, Trease and Evans' pharmacognosy, 13th ed. Bailliere Tindall: London, Philadelphia, 1989.

- P. R. Adams, Identification of essential oil components by gas chromatography/mass spectrometry, Allured Pu. 2007.

- M. A Wikler, Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard. CLSI (NCCLS), 2006, 26, M7-A7.

- F. Lewisoscar, D. Mubarakali, C. Nithya, R. Priyanka, V. Gopinath, N. S. Alharbi, N. Thajuddin, Biofouling: The Journal of Bioadhesion and Biofilm One-pot synthesis and anti-biofilm potential of copper nanoparticles (CuNPs) against clinical strains of Pseudomonas aeruginosa, Biofouling, 2015, 31, 379–391.

- Y. L. Tang, Y. H. Shi, W. Zhao, G. Hao, G. W. Le, Insertion mode of a novel anionic antimicrobial peptide MDpep5 (Val-Glu-Ser-Trp-Val) from Chinese traditional edible larvae of housefly and its effect on surface potential of bacterial membrane, Journal of Pharmaceutical and Biomedical Analysis, 2008, 48, 1187–1194.

- T. Ngouana, C. Mbouna, R. Kuipou, M. Tchuenmogne, E. Zeuko'o, V. Ngouana, M. Mallié, S. Bertout, F. Boyom, Potent and Synergistic Extract Combinations from Terminalia Catappa, Terminalia Mantaly and Monodora tenuifolia Against Pathogenic Yeasts, Medicines, 2015, 2, 220–235.

- E. Simionatto, C. Porto, C. Z. Stüker, I. I. Dalcol, U. F. Da Silva, Chemical composition and antimicrobial activity of the essential oil from Aeolanthus suaveolens Mart. ex Spreng, Quimica Nova, 2007, 30, 1923–1925.

- M. Aminzadeh, F. Amiri, E. A. Abadi, K. Mahdevi, S. Fadai, Factors affecting on essential chemical composition of Thymus kotschyanus in Iran, World Applied Sciences Journal, 2010, 8, 847–856.

- M. Cristani, M. D'Arrigo, G. Mandalari, F. Castelli, M. G. Sarpietro, D. Micieli, V. Venuti, G. Bisignano, A. Saija, D. Trombetta, Interaction of four monoterpenes contained in essential oils with model membranes: Implications for their antibacterial activity, Journal of Agricultural and Food Chemistry, 2007, 55, 6300–6308.

- M. Kong, X. G. Chen, C. S. Liu, C. G. Liu, X. H. Meng, L. J. Yu, Antibacterial mechanism of chitosan microspheres in a solid dispersing system against E. coli, Colloids and Surfaces B: Biointerfaces, 2008, 65, 197–202.

- M. M. Bazargani, J. Rohloff, Antibiofilm activity of essential oils and plant extracts against Staphylococcus aureus and Escherichia coli biofilms, Food Control, 2016, 61, 156–164.

- S. W. Kim, I. M. Chang, K. B. Oh, Inhibition of the Bacterial Surface Protein Anchoring Transpeptidase Sortase by Medicinal Plants, Bioscience, Biotechnology and Biochemistry, 2002, 66, 2751–2754.

- S. Mizunaga, T. Kamiyama, Y. Fukuda, M. Takahata, J. Mitsuyama, Influence of inoculum size of Staphylococcus aureus and Pseudomonas aeruginosa on in vitro activities and in vivo efficacy of fluoroquinolones and carbapenems, Journal of Antimicrobial Chemotherapy, 2005, 56, 91–96.

- F. Bakkali, S. Averbeck, D. Averbeck, M. Idaomar, Biological effects of essential oils- A review, Food and Chemical Toxicology, 2008, 46, 446–475.

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Published

02-02-2021

Issue

Vol. 11 No. 1 (2021)

Section

Medicinal Chemistry

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