Cover Image

Spectrophotometric determination of Uranium through complex formation with roxarsone

Nashat Mohamed Alanwar Abd alaty


A new sensitive, accurate, and non-extractive spectrophotometric method was developed for the rapid determination of Uranium in pure form and Uranium ore using roxarsone. The procedure was based on the complex formation between Uranium (VI) and roxarsone. This showed maximum absorption at λmax 395 nm with a linear relationship in the concentration range from (20-100 µg mLˉ¹) with a molar absorptivity 9.57×103(1mol-1 cm-1). Inductively Coupled Plasma mass spectroscopy (ICP-MS) was used in the study of the interferences caused by some metallic ions, which were effectively masked by tartaric acid and diethylenetriaminepenta-acetic acid (DTPA). The method holds its accuracy and precision well when applied to the determination of the studied Uranium in its pure form and Uranium ore. 


Full Text:



- P. Subrahmanyam, B. Krishnapriya, K. Suvardhan, D. Rekha, Y. Suneeta, B. Jayaraj, P. Chiranjeevi, Simple, selective, and sensitive spectrophotometric determination of fenitrothion using a novel chromogenic reagent, Journal of Hazardous Materials, 2007, 146, 51-57.

- H. G. Daabees, A new sensitive spectrophotometry method for the analysis of some antihistamines, Spectroscopy Letters, 1999, 32, 913-930.

- T. T. D. Tien, P. N. D. Uyen, T. B. T. Huong, T. N. Trang, Effect of electrostatic interaction between fluoxetine and lipid membranes on the partitioning of fluoxetine investigated using second derivative spectrophotometry and FTIR, Chemistry and Physics of Lipids, 2017, 207,


- W. Wei, W. Min, K. Xuejun, W. Ping, L. Songqin, Spectrophotometry study of the interaction of hyaluronic acid with methylene blue and its analytic application, Analytical Letters, 2008, 41, 599-607.

- H. A. El Nahas, M. G. El Feky, H. I. Mira, Mineralogy, m-type tetrad effect, and radioactivity of altered granites at the g. abu garadi shear zone, central-eastern desert, Egypt, Chinese Jor. of Geochem., 2011, 30, 153-164.

- M. F. Cheira, H. I. Mira, A. K. Sakr, S. A. Mohamed, Adsorption behaviors of Uranium (vi) from the acid solution using treated waste clay, Nucl. Sci. and Techniques, 2019, 30, 156-174.

- A. H. Abd El Hameed, W. E. Eweda, K. A. Abou-Taleb, H. I. Mira, Biosorption of Uranium, and heavy metals using some local fungi isolated from phosphatic fertilizers, Annal of Agricultural Sci., 2015, 60, 345-351.

- O. Fujino, S. Umetani, E. Ueno, K. Shigeta, T. Matsuda, Determination of Uranium and thorium in apatite minerals by inductively coupled plasma atomic emission spectrometry with solvent extraction separation into diisobutyl ketone, Anal. Chim. Acta, 2000, 420, 65-71.

- J. G. Gupta, N. B. Bertrand, Direct ICP-MS determination of trace and ultra-trace elements in geological materials after decomposition in a microwave oven. I. Quantitation of Y, Th, U, and the lanthanides, Talanta, 1995, 42, 1595-1607.

- S. Joannon, P. Telouk, C. Pin, Determination of U and Th at ultra-trace levels by isotope dilution inductively coupled plasma mass spectrometry using a geyser-type ultrasonic nebulizer: application to geological samples, Spectrochim. Acta B, 1997, 52, 1783-1789.

- E. H. Borai, A. S. Mady, Separation, and quantification of 238U, 232Th, and rare earth in monazite samples by ion chromatography coupled with on-line flow scintillation detector, Appl. Radiat. Isot, 2002, 57, 463-469.

- B. Liu, L. Liu, J. Cheng, Separation and determination of thorium, Uranium, and mixed rare-earth elements as their U.V./Vis absorbing complexes by capillary zone electrophoresis, Talanta, 1998, 47, 291-299.

- Y. Hirano, Y. Ogawa, K. Oguma, Simultaneous spectrophotometric determination of Uranium and thorium by flow injection analysis using selective masking Anal. Sci., 2003, 19, 303-307.

- N. Dufoura, J. Dumazert, E. Barat, G. H. V. Bertrand, F. Carrel, T. Dautremer, F. Lainea, A. Sari, Measurement of low-activity uranium contamination by gamma-ray spectrometry for nuclear decommissioning, Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors, and Associated Equipment, 2020, 951, 162976.

- Z. Marczenko, Separation and Spectrophotometric Determination of Element, Chichester: E. Horwood; New York: Halsted Press, 1986.

- H. Onishi, Photometric Determination of Traces of Metals Fourth Edition Part I.I.B.: Individual Metals, Magnesium to Zirconium, A Wiley-Interscience Publication, New York, 1989, 411-418.

- H. D. Chapman, Z. B. Johnson, use of antibiotics and roxarsone in broiler chickens in the USA: analysis for the years 1995 to 2000, Poultry Science, 2002, 81, 356-364.

- J. Edmonds, K. Francesconi, Transformations of arsenic in the marine environment, Experientia, 1987, 43, 553-557.

- K. Elgendy, A. El-didamony, B. Abd El-wahaab, Analytical applications using the spectrophotometric technique for the determination of Uranium (VI), samarium(III) and cerium(III) by a new organic reagent, Journal of the Iranian Chemical Society, 2020, 1-11.

- K. Lutfullah, N. A. Mohd, R. Nafisur, N. H. A. Syed, Optimized and validated spectrophotometric method for the determination of Uranium (VI) via complexation with meloxicam, Journal of Hazardous Materials. 2008, 155, 261-268.

- S. G. Leonardo, C. S. C. Antonio, L. C. F. Sergio, L. F. Maria, S. C. Marcelo, Spectrophotometric determination of Uranium using

-(2-thiazolylazo)-p-cresol (tac) in the presence of surfactants, J. Braz. Chem. Soc., 1999, 10, 519-522.

- N. Ali, G. Nahid, G. Mohammad, E. Ali, Simultaneous Spectrophotometric Determination of Uranium and Thorium Using Arsenazo III by H‐Point Standard Addition Method and Partial Least Squares Regression, Journal of the Chinese Chemical Society, 2007, 54, 411-418.

- S. O. Adel, E. Adel, A. S. Medhat, E. A. Alaa, Bulletin of the Chemists and Technologists of Macedonia. 2007, 24, 11-19.

- N. S. B. Singh, S. V. Mohan, A spectrophotometric method for uranium determination, Journal of Radioanalytical and Nuclear Chemistry, 1979, 52, 319–327.

- D. R. Do Carmo, J. F. de Andrade, O. M. Guimares, Spectrophotometric determination of Uranium through uranyl, azide system, Analytical Letters, 1995, 28, 1897-1911.

- K. Danchana, C. T. de Souza, E. Palacio, V. Cerda, Multisyringe flow injection analysis for the spectrophotometric determination of Uranium (VI) with 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol, Microchemical Journal, 2019, 150, 104148.

- V. S. A. Devi, V. K. Reddy, 2-Hydroxy-1-naphthaldehyde-p-hydroxybenzoichydrazone: a new chromogenic reagent for the determination of thorium (iv) and Uranium (vi), Journal of Chemistry, 2013, 697379.

- C. E. Crouthamel, C. E. Johnson, Spectrophotometric determination of Uranium by thiocyanate method in acetone medium, Anal. Chem., 1952, 24, 1780-1783.

- R. Ali, A. Suhail, M. Ejaz, Spectrophotometric determination of Uranium (VI) with 7-iodo-8-hydroxyquinol ine-5-sulfonic acid (ferron), Journal of Radioanalytical and Nuclear Chemistry, 1979, 49, 205.

- M. A. El-Ries, Spectrophotometric determination of piroxicam and tenoxicam in pharmaceutical preparations using uranyl acetate as a chromogenic agent, Analytical Letters, 1998, 31, 793-807.

- A. T. Elham, Y. H. Nagiba, A. A. Fahima, E. A. Laila, Fluorimetric Determination of Some Sulfur-Containing Compounds Through Complex Formation with Terbium (Tb+3) and Uranium (U+3), Journal of Fluorescence, 2007, 17, 293–300.

- H. K. Line, E. N. Peter, I. J. Christian, B. K. Birthe, E. M. Niels, Phosphate Selective Uranyl Photo‐Affinity Cleavage of Proteins.

Determination of Phosphorylation Sites, ChemBioChem J., 2008, 9, 2377-2381.

- U. Saha, A. K. Sen, T. K. Das, S. K. Bhowal, Spectrophotometric determination of tetracyclines in pharmaceutical preparations, with uranyl acetate, Talanta, 1990, 37, 1193-1196.

- V. A. Madalina, L. A. Gabriela, T. Rosua, M. Negoiu, A. Eva-Ruxandra, R. Jenny, Copper(II), and uranyl(II) complexes with acylthiosemicarbazide: Synthesis, characterization, antibacterial activity and effects on the growth of promyelocytic leukemia cells HL-60, European Journal of Medicinal Chemistry, 2009, 44, 3323-3329.

- U. Saha, A. K. Sen, T. K. Das, Spectrophotometric determination of clioquinol and diodoquin in pharmaceutical preparations using uranyl acetate as a chromogenic agent, Analyst, 1988, 113, 1653-1655.

- A. C. Parekh, D. H. Jung, Cholesterol determination with ferric acetate-uranium acetate and sulfuric acid-ferrous sulfate reagents, Anal. Chem., 1970, 42, 1423-1427.

- Y. Hirano, Y. Ogawa, K. Oguma, Simultaneous Spectrophotometric Determination of Uranium and thorium by flow injection analysis using selective masking, Analytical Sciences, 2003, 19, 303-307.

- M. H. Gabb, W. E. Latchem, A Handbook of Laboratory Solutions, NewYork, Chemical Publishing Company Inc., 1968.

- J. Rose, Advanced physicochemical experiments, Pitman, London, England, 1964, 54.



  • There are currently no refbacks.

Copyright (c) 2020 Mediterranean Journal of Chemistry