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Lead uptake, flavonoids, and proline relationship in Atriplex nummularia growing in a galena mining area

Anass Merzougui, Lasky Mariama, Fatima Ezzahrae Hamid, Mohamed Lachgar, Abdellah El Anssari, Driss Mrani


Phytoremediation is an emerging cost-effective remediation technology that uses plants to remove metals from contaminated soils. Average levels of Lead (Pb), total flavonoids content (TFC), and proline were assessed in 108 samples of Atriplex nummularia growing around an active mining area in southeastern Morocco.

From January to December 2018, three different locations were sampled within a radius of 0 to 800 meters from the central ore storage area to monitor the evolution of these content levels. These results were compared to a control group of plants.

Lead uptake ranged between 16-270 ppm and was found to be irregular during the sampling time frame or within the geographic location. Measurement results showed the expected effect of metal uptake on flavonoids and proline content.  Thus, high levels of lead are likely correlated with increasing proline (r = 0.949**) and decreasing flavonoids (r = 0.972**) concentrations, respectively.

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- T. Amari, T. Ghnaya, C. Abdelly, Nickel, cadmium and lead phytotoxicity and potential of halophytic plants in heavy metal extraction, South Afr J Bot, 2017, 111, 99–110.

- A. Piechalak, B. Tomaszewska, D. Baralkiewicz, A. Malecka, Accumulation and detoxification of lead ions in legumes, Phytochemistry, 2002, 60, 153–162.

- R. Gopal, A. H. Rizvi, Excess lead alters growth, metabolism and translocation of certain nutrients in radish, Chemosphere, 2008, 70, 1539–1544.

- M. Masidur Alam, S. Hayat, B. Ali, A. Ahmad, Effect of 28-homobrassinolide treatment on nickel toxicity in Brassica juncea, Photosynthetica, 2007, 45, 139–142.

- J. Brunet, G. Varrault, Y. Zuily-Fodil, A. Repellin, Accumulation of lead in the roots of grass pea (Lathyrus sativus L.) plants triggers systemic variation in gene expression in the shoots, 2009, 8.


- S. Rostami, A. Azhdarpoor, The application of plant growth regulators to improve phytoremediation of contaminated soils: A review, Chemosphere, 2019, 220, 818–827.

- D. C. ADRIANO, TRACE ELEMENTS IN TERRESTRIAL ENVIRONMENTS: biogeochemistry, bioavailability, and risks of... metals., SPRINGER: S.l., 2002.

- E. Meers, A. Ruttens, M. J. Hopgood, D. Samson, F. M. G. Tack, Comparison of EDTA and EDDS as potential soil amendments for enhanced phytoextraction of heavy metals, Chemosphere, 2005, 58, 1011–1022.

- S. Tandy, R. Schulin, B. Nowack, The influence of EDDS on the uptake of heavy metals in hydroponically grown sunflowers, Chemosphere, 2006, 62, 1454–1463.

- S. S. Kachout, A. B. Mansoura, R. Mechergui, J. C. Leclerc, M. N. Rejeb, Z. Ouerghi, Accumulation of Cu, Pb, Ni and Zn in the halophyte plant Atriplex grown on polluted soil, J Sci Food Agric, 2012, 92, 336–342.

- T. Amari, T. Ghnaya, C. Abdelly, Nickel, cadmium and lead phytotoxicity and potential of halophytic plants in heavy metal extraction, South Afr J Bot, 2017, 111, 99–110.

- B. Nedjimi, Y. Daoud, Cadmium accumulation in Atriplex halimus subsp. schweinfurthii and its influence on growth, proline, root hydraulic conductivity and nutrient uptake, Flora - Morphol Distrib Funct Ecol Plants, 2009, 204, 316–324.

- M. S. Stanković, M. Petrović, D. Godjevac, Z. D. Stevanović, Screening inland halophytes from the central Balkan for their antioxidant activity in relation to total phenolic compounds and flavonoids: Are there any prospective medicinal plants?, J Arid Environ, 2015, 120, 26–32.


- S. Albu, Potential for the use of ultrasound in the extraction of antioxidants from Rosmarinus officinalis for the food and pharmaceutical industry, Ultrason Sonochem, 2004, 11, 261–265.

- F. Pourmorad, S. J. Hosseinimehr, N. Shahabimajd, Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants, 4.

- E. Wieteska, A. Zióek, A. Drzewińska, Extraction as a method for preparation of vegetable samples for the determination of trace metals by atomic absorption spectrometry, Anal Chim Acta, 1996, 330, 251–257.

- K. Jankowski, A. G. Ciepiela, J. Jankowska, W. Szulc, R. Kolczarek, J. Sosnowski, B. Wiśniewska-Kadżajan, E. Malinowska, E. Radzka, W. Czeluściński, J. Deska, Content of lead and cadmium in aboveground plant organs of grasses growing on the areas adjacent to a route of big traffic, Environ Sci Pollut Res, 2015, 22, 978–987.

- S. Candir, I. Narin, M. Soylak, Ligandless cloud point extraction of Cr(III), Pb(II), Cu(II), Ni(II), Bi(III), and Cd(II) ions in environmental samples with Tween 80 and flame atomic absorption spectrometric determination, Talanta, 2008, 77, 289–293.

- P. Theriappan, A. Gupta, P. Dhasarrathan, Accumulation of Proline under Salinity and Heavy metal stress in Cauliflower seedlings, J Appl Sci Environ Manag, 2011, 15 doi:10.4314/jasem.v15i2.68497.

- Alia, P. P. Saradhi, Proline Accumulation Under Heavy Metal Stress, J Plant Physiol, 1991, 138, 554–558.

- L. S. Bates, R. P. Waldren, I. D. Teare, Rapid determination of free proline for water-stress studies, Plant Soil, 1973, 39, 205–207.

- M. Atanasova, F. Ribarova, Phénols et flavonoïdes totaux dans les extraits secs des feuilles des bouleaux argentés bulgares (Betula pendula), , 2009, , 6.

- O. Belkheiri, M. Mulas, The effects of salt stress on growth, water relations and ion accumulation in two halophytes Atriplex species, Environ Exp Bot, 2013, 86, 17–28.

- A.-N. Liu, L.-L. Wang, H.-P. Li, J. Gong, X.-H. Liu, Correlation Between Posttraumatic Growth and Posttraumatic Stress Disorder Symptoms Based on Pearson Correlation Coefficient: A Meta-Analysis, J Nerv Ment Dis, 2017, 205, 380–389.

- A. Michalak, Phenolic Compounds and Their Antioxidant Activity in Plants Growing under Heavy Metal Stress, 8.

- K. Keilig, J. Ludwig-Müller, Effect of flavonoids on heavy metal tolerance in Arabidopsis thaliana seedlings, Bot Stud, 2009, 50, 8.



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