Cover Image

Evaluation of Antioxidant Activity of fresh Lemon (Citrus lemon L.) peel in Marrakech, Kenitra cities of Morocco and Taiz of Yemen

Khaled Abdu, Rahma Erahioui, Amina Moutawalli, Ahmed Zahidi, Khadija Khedid, Said Ibn Ahmed

Abstract


The principal objective of this study was to appraise the variation of antioxidant activity, total phenols, and total flavonoids of fresh lemon peel from regions located Marrakech and Kenitra in Morocco and a region Taiz in Yemen. The test of polyphenol was performed from extracts that were measured using the Folin-Ciocalteu technique. Also, an analysis of Flavonoid was performed of was measured by using the aluminum chloride. The Free radical scavenging activity of lemon extracts was measured by 2,2′-Diphenyl-1-picrylhydrazyl hydrate (DPPH).

The DPPH radical scavenging activity of ethanolic extract of fresh lemon peel found to be highest at 200μl concentration, which was 77. 15 %; 67. 88 %; 37.74%. Through it, 50% inhibition (IC50) was obtained to be 92. 04; 153. 55 and 215.407 μg/ml in Marrakech, Kenitra, and Taiz, respectively. As well, the values radical scavenging activity of methanolic extract of the same concentration, which are 82, 77 %, 53, 33 %, and 47,5%. Through it, 50% inhibition (IC50) was obtained to be 145. 6; 179. 17 and 274.899 μg/ml in Marrakech, Kenitra, and Taiz, respectively. But regarding the values radical scavenging activity of essential oil of the same concentration, which is 63.77 %, 42.19 %, and 80.25%. Through it, 50% inhibition (IC50) was obtained to be 155.54; 271.158 and 101.31 μg/ml in Marrakech, Kenitra, and Taiz, respectively. According to the results obtained in our study, the antioxidant activity increases with increased polyphenols and IC50 deficiency in Marrakech, Kenitra, and Taiz.


Full Text:

PDF

References


- S. Zahoor, F. Anwar, T. Mehmood, B. Sultana, R. Qadir, Variation In Antioxidant Attributes And Individual Phenolics Of Citrus Fruit Peels In Relation To Different Species And Extraction Solvents, Journal of the Chilean Chemical Society, 2016, 61 (2), 2884–2889.

- R. Ns, H. Sm, Estimation of Phytochemical Content and Antioxidant Activity of Some Selected Traditional Indian Medicinal Plants, Indian J Pharm Sci, 2011, 73 (2), 146–151.

- A. Owosini, A. Ayansina, O. Amjo, In vitro assessment of the antimicrobial activities of leaf and stem extracts of Alchorneacordifolia, J Appl Sci Environ Manag, 2015, 19(2), 303-308.

- J. A. Del Rıo, M. D. Fuster, P. Gómez, I. Porras, A. Garc ́ ıa-Lidón, A. Ortuño, Citruslimon: a source of flavonoids of pharmaceutical interest, Food Chem, 2004, 84 (3), 457–461.

- M. Boluda-Aguilar, A. López-Gómez, Production of Bioethanol by Fermentation of Lemon (Citrus Limon L.) Peel Wastes Pretreated with Steam Explosion, Industrial crops and products, 2013, 41, 188–197.

- Y. Chen, T. J. Barzee, R. Zhang, Z. Pan, Citrus, Integrated Processing Technologies for Food and Agricultural By-Products, Academic Press, 2019, 217–242.

- R. Apak, K. Güçlü, B. Demirata, M. Özyürek, S. Çelik, B. Bektaşoğlu, K. Berker, D. Özyurt, Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay, Mol, 2007, 12 (7), 1496-1547.

- A. Gil-Izquierdo, M. T. Riquelme, I. Porras, F. Ferreres, Effect of the rootstock and interstock grafted in lemon tree (Citrus limon (L.) Burm.)on the flavonoid content of lemon juice, J. Agric. Food Chem, 2004, 52 (2), 324–331.

- Z. Zou, W. Xi, Y. Hu, C. Nie, Z. Zhou, Antioxidant activity of Citrus fruits, Food Chemistry, 2016, 196, 885–896.

- J. U. Ewansiha, S. A. Garba, M. Galadima, S. Y. Daniyan, M. B. Busari, Therapeutic Potency of Citrus Limon (L) Burm. F. (Lemon) Peel Extract Against Some Disease-Causing Microorganisms, Int J Res Stud Biosci, 2020, 4(11), 30-39.

- R. Adams, Identification of Essential Oil Components by Gas Chromatography/Quadrupole Mass Spectroscopy, Carol Stream, 2005, 16, 1902-1903.

- E. Lister, P. Wilson, Measurement of total phenolicsandABTS assay for antioxidant activity (personal communication), Crop Research Institute, 2001, 36, 718-744.

- J. D. Ordon, M. A. Gomez, M. I. Vattuone, J. D. Ordon, M. A. Gomez, M. I. Vattuone, Antioxidant activities of Sechiumedule, Swartz extracts, Food Chemistry, 2006, 97, 452–458.

- B. Huang, H. Ke, J. He, X. Ban, H. Zeng, Y. Wang, Extracts of Haleniaelliptica exhibit antioxidant properties in vitro and in vivo, Food Chem Toxicol, 2001, 49, 185-190.

- A. Jahanban-Esfahlan, A. Ostadrahimi, M. Tabibiazar, R. Amarowicz, A Comparative Review on the Extraction, Antioxidant Content and Antioxidant Potential of Different Parts of Walnut (JuglansRegia L.) Fruit and Tree, Molecules, 2019, 24 (11), 2133.

- A. Hegazy, M. Ibrahium, Antioxidant Activities of Orange Peel Extracts, World Applied Sciences Journal 2012, 18 (5), 684-688.

- X. Liu, M. Dong, X. Chen, M. Jiang, X. Lv, G. Yan, Antioxidant activity and phenolics of an endophytic Xylaria sp. From Ginkgo biloba. Food Chem, 2007, 105 (2), 548–554.

- P. Kuppusamy, M. M. Yusoff, N. R. Parine, N. Govindan, Evaluation of In-Vitro Antioxidant and Antibacterial Properties of Commelina Nudiflora L. Extracts Prepared by Different Polar Solvents, Saudi Journal of Biological Sciences 2015, 22 (3), 293–301.




DOI: http://dx.doi.org/10.13171/mjc10602006261379ka

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Mediterranean Journal of Chemistry