Extraction of bioethanol from cassava root and cocoyam using saccharomyces cerevisiae strain with differing H2SO4 concentrations
DOI:
https://doi.org/10.13171/mjc02503011822kamaraAbstract
Bioethanol (CH3CH2OH), or ethyl alcohol, is a renewable biofuel produced by fermentation of various agricultural products. Cassava and cocoyam tubers were utilized as starting materials to extract ethanol. The two raw materials were separately weighed, peeled, washed in separate bowls, ground into a paste, and then mixed with distilled water. The samples were decanted and pressed with a cloth to remove the remaining water. The two samples were dried for 24 hours and then pre-treated in an autoclaved chamber for 15 minutes at 1200C. The two samples were hydrolyzed in five different concentrations of dilute H2SO4 and then placed in a water bath for 5 hours, followed by adding NaOH to reduce the pH of the hydrolyzed starch solution. A culture solution (S. Cerevisiae) and broth media were separately prepared. The culture solution was added to the media solutions and incubated for 72 hours at 300C and 200 rpm. The samples were conditioned in a shaking incubator and fermented for 7 days. The samples were distilled in a 500 mL round bottom flask at 780C for 3 hours to extract ethanol from the solution. This procedure was done for cassava and cocoyam samples one at a time. The ethanol from cassava and cocoyam at different acid concentrations was measured and recorded. The results show that the ethanol yield from cassava at different acid concentrations was greater than that of cocoyam.
References
References
C. Zou, Q. Zhao, G. Zhang, and B. Xiong, "Energy revolution: From a fossil energy era to a new energy era," Natural Gas Industry B, vol. 3, pp. 1-11, 2016.
R. York and S. E. Bell, "Energy transitions or additions?: Why a transition from fossil fuels requires more than the growth of renewable energy," Energy Research & Social Science, vol. 51, pp. 40-43, 2019.
A. Callegari, S. Bolognesi, D. Cecconet, and A. G. Capodaglio, "Production technologies, current role, and future prospects of biofuels feedstocks: A state-of-the-art review," Critical Reviews in Environmental Science and Technology, vol. 50, pp. 384-436, 2020.
V. G. Yadav, G. D. Yadav, and S. C. Patankar, "The production of fuels and chemicals in the new world: critical analysis of the choice between crude oil and biomass vis-à-vis sustainability and the environment," Clean technologies and environmental policy, vol. 22, pp. 1757-1774, 2020.
B. Gabrielle, L. Bamière, N. Caldes, S. De Cara, G. Decocq, F. Ferchaud, et al., "Paving the way for sustainable bioenergy in Europe: technological options and research avenues for large-scale biomass feedstock supply," Renewable and Sustainable Energy Reviews, vol. 33, pp. 11-25, 2014.
S. Niphadkar, P. Bagade, and S. Ahmed, "Bioethanol production: insight into past, present and future perspectives," Biofuels, vol. 9, pp. 229-238, 2018.
E. Bertrand, L. P. Vandenberghe, C. R. Soccol, J.-C. Sigoillot, and C. Faulds, "First generation bioethanol," Green fuels technology: biofuels, pp. 175-212, 2016.
H. Zabed, J. Sahu, A. Suely, A. Boyce, and G. Faruq, "Bioethanol production from renewable sources: Current perspectives and technological progress," Renewable and Sustainable Energy Reviews, vol. 71, pp. 475-501, 2017.
M. Guo, W. Song, and J. Buhain, "Bioenergy and biofuels: History, status, and perspective," Renewable and sustainable energy reviews, vol. 42, pp. 712-725, 2015.
M. Vohra, J. Manwar, R. Manmode, S. Padgilwar, and S. Patil, "Bioethanol production: Feedstock and current technologies," Journal of Environmental Chemical Engineering, vol. 2, pp. 573-584, 2014.
L. A. J. Letti, E. B. Sydney, J. C. de Carvalho, L. P. de Souza Vandenberghe, S. G. Karp, A. L. Woiciechowski, et al., "Roles and impacts of bioethanol and biodiesel on climate change mitigation," in Biomass, biofuels, biochemicals, ed: Elsevier, 2022, pp. 373-400.
A. G. B. Ozdingis and G. Kocar, "Current and future aspects of bioethanol production and utilization in Turkey," Renewable and Sustainable Energy Reviews, vol. 81, pp. 2196-2203, 2018.
K. T. Tan, K. T. Lee, and A. R. Mohamed, "Role of energy policy in renewable energy accomplishment: the case of second-generation bioethanol," in Renewable Energy, ed: Routledge, 2018, pp. Vol3_346-Vol3_358.
S. Litvak and O. Litvak, "Some aspects of reducing greenhouse gas emissions by using biofuels," Journal of Ecological Engineering, vol. 21, pp. 198-206, 2020.
T.-D. Hoang and N. Nghiem, "Recent developments and current status of commercial production of fuel ethanol," Fermentation, vol. 7, p. 314, 2021.
M. Krajang, K. Malairuang, J. Sukna, K. Rattanapradit, and S. Chamsart, "Single-step ethanol production from raw cassava starch using a combination of raw starch hydrolysis and fermentation, scale-up from 5-L laboratory and 200-L pilot plant to 3000-L industrial fermenters," Biotechnology for Biofuels, vol. 14, pp. 1-15, 2021.
G. Naoura, Y. Emendack, N. Baloua, K. Vom Brocke, M. A. Hassan, N. Sawadogo, et al., "Characterization of semi-arid Chadian sweet sorghum accessions as potential sources for sugar and ethanol production," Scientific Reports, vol. 10, p. 14947, 2020.
R. A. Cripwell, L. Favaro, M. Viljoen-Bloom, and W. H. van Zyl, "Consolidated bioprocessing of raw starch to ethanol by Saccharomyces cerevisiae: Achievements and challenges," Biotechnology Advances, vol. 42, p. 107579, 2020.
J. Dees, K. Oke, H. Goldstein, S. T. McCoy, D. L. Sanchez, A. Simon, et al., "Cost and life cycle emissions of ethanol produced with an oxyfuel boiler and carbon capture and storage," Environmental Science & Technology, vol. 57, pp. 5391-5403, 2023.
A. Singh, P. Prajapati, S. Vyas, V. K. Gaur, R. Sindhu, P. Binod, et al., "A comprehensive review of feedstocks as sustainable substrates for next-generation biofuels," BioEnergy Research, vol. 16, pp. 105-122, 2023.
A. H. Hirani, N. Javed, M. Asif, S. K. Basu, and A. Kumar, "A review on first-and second-generation biofuel productions," Biofuels: greenhouse gas mitigation and global warming: next generation biofuels and role of biotechnology, pp. 141-154, 2018.
B. V. Ayodele, M. A. Alsaffar, and S. I. Mustapa, "An overview of integration opportunities for sustainable bioethanol production from first-and second-generation sugar-based feedstocks," Journal of Cleaner Production, vol. 245, p. 118857, 2020.
A. G. Capodaglio and S. Bolognesi, "Ecofuel feedstocks and their prospects," in Advances in eco-fuels for a sustainable environment, ed: Elsevier, 2019, pp. 15-51.
A. Datta, A. Hossain, and S. Roy, "An overview on biofuels and their advantages and disadvantages," 2019.
I. Bioenergy, "From 1st-to 2nd-Generation BioFuel technoloGies," An overview of current industry and RD&D activities. IEA-OECD, 2008.
M. Hiloidhari, N. Bhuyan, N. Gogoi, D. Seth, A. Garg, A. Singh, et al., "Agroindustry wastes: biofuels and biomaterials feedstocks for sustainable rural development," in Refining Biomass Residues for Sustainable Energy and Bioproducts, ed: Elsevier, 2020, pp. 357-388.
R. A. Olawoyin, "Comparative Studies of Bioethanol Production From Manihot esculenta Peels Using Aspergillus niger and Saccharomyces cerevisiae in Submerged Fermentation," Kwara State University (Nigeria), 2023.
C. C. Ogbu and S. N. Okey, "Agro-industrial waste management: the circular and bioeconomic perspective," Agricultural Waste-New Insights, 2023.
M. Emetere and W. O. Joel, "Biofuel Commercialization in Developing Countries: Readiness and Prospects," Journal of Renewable Energy and Environment, vol. 10, pp. 119-130, 2023.
S. Xia, L. Zhang, A. Davletshin, Z. Li, J. You, and S. Tan, "Application of polysaccharide biopolymer in petroleum recovery," Polymers, vol. 12, p. 1860, 2020.
M. U. Adikwu and C. O. Esimone, "Biopolymers in drug delivery: recent advances and challenges," 2010.
P. Sakthivel, K. Subramanian, and R. Mathai, "Indian scenario of ethanol fuel and its utilization in automotive transportation sector," Resources, Conservation and Recycling, vol. 132, pp. 102-120, 2018.
M. Di Girolamo, M. Brianti, and M. Marchionna, "Octane enhancers," Handbook of Fuels: Energy Sources for Transportation, pp. 403-430, 2021.
S. Taneja, P. Singh, A. Sharma, and G. Singh, "Use of alcohols and biofuels as automotive engine fuel," Energy Systems and Nanotechnology, pp. 161-183, 2021.
J. Badia, E. Ramírez, R. Bringué, F. Cunill, and J. Delgado, "New octane booster molecules for modern gasoline composition," Energy & Fuels, vol. 35, pp. 10949-10997, 2021.
T. M. Abdellatief, M. A. Ershov, V. D. Savelenko, V. M. Kapustin, U. A. Makhova, N. A. Klimov, et al., "Advanced progress and prospects for producing high-octane gasoline fuel toward market development: State-of-the-art and outlook," Energy & Fuels, vol. 37, pp. 18266-18290, 2023.
T. M. Abdellatief, M. A. Ershov, A. E. Makhmudova, V. M. Kapustin, U. A. Makhova, N. A. Klimov, et al., "Novel variants conceptional technology to produce eco-friendly sustainable high octane-gasoline biofuel based on renewable gasoline component," Fuel, vol. 366, p. 131400, 2024.
M. Frosi, A. Tripodi, F. Conte, G. Ramis, N. Mahinpey, and I. Rossetti, "Ethylene from renewable ethanol: Process optimization and economic feasibility assessment," Journal of industrial and engineering chemistry, vol. 104, pp. 272-285, 2021.
B. P. Singh, S. Agnihotri, G. Singh, and V. K. Gupta, "Postharvest Management of Fresh Produce: Recent Advances," 2023.
J. A. Castañeda-Ayarza and L. A. B. Cortez, "Final and B molasses for fuel ethanol production and some market implications," Renewable and Sustainable Energy Reviews, vol. 70, pp. 1059-1065, 2017.
N. N. Iderus, M. Shaha, E. Mohameda, A. S. Mohd Kassima, and M. Abdul Hamid, "Bioethanol production from cassava and sugar cane molasses," 2022.
E. W. Gabisa, C. Bessou, and S. H. Gheewala, "Life cycle environmental performance and energy balance of ethanol production based on sugarcane molasses in Ethiopia," Journal of Cleaner Production, vol. 234, pp. 43-53, 2019.
E. E. Elemike, O. C. Oseghale, and A. C. Okoye, "Utilization of cellulosic cassava waste for bio-ethanol production," Journal of Environmental Chemical Engineering, vol. 3, pp. 2797-2800, 2015.
B. Escaramboni, "Development of the bioprocess of ethanol production from starch residues using amylolytic enzymes produced by Rhizopus oligosporus CCT 3762," 2019.
B. Escaramboni, E. G. F. Núñez, A. F. A. Carvalho, and P. de Oliva Neto, "Ethanol biosynthesis by fast hydrolysis of cassava bagasse using fungal amylases produced in optimized conditions," Industrial Crops and Products, vol. 112, pp. 368-377, 2018.
D. G. Martinez, A. Feiden, R. Bariccatti, and K. R. de Freitas Zara, "Ethanol production from waste of cassava processing," Applied Sciences, vol. 8, p. 2158, 2018.
B. D. Mignouna, P. L. Kumar, D. Coyne, R. Bandyopadhyay, A. Ortega-Beltran, R. Bhattacharjee, et al., "Identifying and managing plant health risks for key African crops: yam, taro and cocoyam," in Critical issues in plant health, ed: Burleigh Dodds Science Publishing, 2019, pp. 213-228.
C. Ukwu, A. Yahaya, P. Okere, N. Aladi, V. Odoemenam, H. Obikaonu, et al., "The production, uses, nutritional and anti-nutritional characteristics of cocoyam as a potential feed ingredient in the tropics: a review," Nigerian Journal of Animal Science, vol. 24, pp. 91-111, 2022.
V. Lebot, A. Ivančič, and F. Lawac, "Cocoyam (Xanthosoma sagittifolium (L.) Schott) genetic resources and breeding: a review of 50 years of research efforts," Genetic Resources and Crop Evolution, pp. 1-20, 2024.
J. Quero-Garcia, A. Ivancic, and V. Lebot, "Taro and cocoyam," Root and tuber crops, pp. 149-172, 2010.
T.-T. Chang, "Origin, domestication, and diversification," Rice: origin, history, technology, and production, pp. 3-26, 2003.
I. Nwankwo, "A REVIEW OF COCOYAM RESEARCH PROGRAMME ACTIVITIES IN NATIONAL ROOT CROPS RESEARCH INSTITUTE, UMUDIKE SINCE 1923," CENTENNIAL, p. 240, 2023.
J. Muthoni and H. Shimelis, "Minor root and tuber crops in Africa: Cocoyams ('Colocasia esculenta and Xanthosoma sagittifolium')," Australian Journal of Crop Science, vol. 17, pp. 653-663, 2023.
R. Aidoo, S. Boakye-Achampong, P. Wie, B. G. Appiah, and K. Nkrumah, "Root and tuber crops in Ghana-an overview," Roots And Tubers In Ghana: Overview And Selected Research Papers, p. 1, 2019.
A. A. Boakye, F. D. Wireko‐Manu, I. Oduro, W. O. Ellis, M. Gudjónsdóttir, and I. S. Chronakis, "Utilizing cocoyam (Xanthosoma sagittifolium) for food and nutrition security: A review," Food Science & Nutrition, vol. 6, pp. 703-713, 2018.
M. U. Khan and B. K. Ahring, "Lignin degradation under anaerobic digestion: Influence of lignin modifications-A review," Biomass and Bioenergy, vol. 128, p. 105325, 2019.
O. C. Amadi, N. C. Onyenma, T. N. Nwagu, C. I. Nnamchi, I. A. Ndubuisi, S. O. Akachukwu, et al., "Total utilization of different parts of wild cocoyam in production of sugar feedstock for bioethanol production; an integrated approach," Bioresource Technology Reports, vol. 12, p. 100550, 2020.
D. Rinaldo, "Carbohydrate and bioactive compounds composition of starchy tropical fruits and tubers, in relation to pre and postharvest conditions: A review," Journal of Food Science, vol. 85, pp. 249-259, 2020.
O. A. Otekunrin, B. Sawicka, A. G. Adeyonu, O. A. Otekunrin, and L. Rachoń, "Cocoyam [Colocasia esculenta (L.) Schott]: exploring the production, health and trade potentials in Sub-Saharan Africa," Sustainability, vol. 13, p. 4483, 2021.
M. P. Cereda, J. Pereira, and L. A. Andrade, "Characterization of underground starchy crops as raw materials: carbohydrates, starch, mucilage, and phenolic compounds," in Starchy Crops Morphology, Extraction, Properties and Applications, ed: Elsevier, 2023, pp. 189-216.
N. N. Uchegbu, C. N. Ude, C. F. Okoyeuzu, O. M. M. Nwadi, W. A. Rasaq, C. U. Ogbonna, et al., "Optimisation of enzymatic fermented glucose production of wild cocoyam starch using response surface methodology," Glob. NEST J, vol. 24, pp. 351-361, 2022.
N. Akhtar, K. Gupta, D. Goyal, and A. Goyal, "Recent advances in pretreatment technologies for efficient hydrolysis of lignocellulosic biomass," Environmental Progress & Sustainable Energy, vol. 35, pp. 489-511, 2016.
Z. Zhou, D. Liu, and X. Zhao, "Conversion of lignocellulose to biofuels and chemicals via sugar platform: an updated review on chemistry and mechanisms of acid hydrolysis of lignocellulose," Renewable and Sustainable Energy Reviews, vol. 146, p. 111169, 2021.
K. Robak and M. Balcerek, "Review of second generation bioethanol production from residual biomass," Food technology and biotechnology, vol. 56, p. 174, 2018.
H. M. Mustafa, D. Salihu, A. Bashir, and A. Ibrahim, "Bio-ethanol production from cassava (Manihot esculenta) waste peels using acid hydrolysis and fermentation process," Science World Journal, vol. 14, pp. 45-50, 2019.
V. E. Efeovbokhan, L. Egwari, E. E. Alagbe, J. T. Adeyemi, and O. S. Taiwo, "Production of bioethanol from hybrid cassava pulp and peel using microbial and acid hydrolysis," BioResources, vol. 14, pp. 2596-2609, 2019.
M. A. Balarabe and O. F. Ibuowo, "Effect of Acid Concentration on the Yield of Bio-ethanol Produced from Corncobs," World, vol. 4, pp. 59-62, 2019.
D.-C. N. Phil, A. Amadi, I. Onuabuchi, and M. Chukwu, "Influence of storage life and variety on the micro-nutrient compositions of stored cocoyam-based products," Food Therapy and Health Care, vol. 4, p. 9, 2022.
C. Ezeonu and N. Ezeonu, "Alternative sources of petrochemicals from readily available biomass and agro-products in Africa: A review," J Pet Environ Biotechnol, vol. 7, p. 2, 2016.
C. Pradeepika, D. J. Kalita, C. Visalakshi Chandra, and K. Hanume Gowda, "Yams and Aroid Crop Waste: Bio Valorization into Bioproducts and Platform Chemicals," in Roots, Tubers, and Bulb Crop Wastes: Management by Biorefinery Approaches, ed: Springer, 2024, pp. 149-181.
F. Baah, B. Maziya-Dixon, R. Asiedu, I. Oduro, and W. O. Ellis, "Nutritional and biochemical composition of D. alata (Dioscorea spp.) tubers," J. Food Agric. Environ, vol. 7, pp. 373-378, 2009.
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