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Bioactive flavonoid from the Commelina obliqua Vahl. and its In vitro anti-inflammatory activity

Kesar Singh, R N Yadava, Ritu Yadav


Commelina obliqua Vahl. This plant is a member of the Commelinaceae family, commonly referred to as the dayflower family. An entirely new bioactive compound recently discovered and identified 3, 5, 7, 3' 4′-pentahydroxy flavone-7-O-L-ribopyranosyl-3′-O-D-galactopyranoside having a melting point 320-22°C, molecular formula C26H28O16, m/z [595-H]- ESI-MS negative mode by different chemical color reactions, chemical decomposition, and spectral analysis 1HNMR, 13CNMR, Electron spray ionization mass spectrometry, Fourier transform infrared spectroscopy. An in vitro anti-inflammatory test revealed significant anti-inflammatory effects besides the degradation of egg albumin. Compound M illustrated the more percentage inhibitory action (123.1974%) of protein denaturation at 500 μg/ml, and Diclofenac sodium demonstrated less inhibitory activity (120.0627%) at the equivalent combination. As a result, Compound M inhibits protein denaturation more effectively than Diclofenac sodium at the same combination. Therefore, compound M may utilize as an anti-inflammatory source.

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K.R. Kirtikar, B.D. Basu, Indian-Medicinal-Plants, Periodical Experts Book Agency, Delhi, 1991, 2534-2536.

J.A. Duke, Database of phytochemical constituents of GRAS herbs and other economic plants, CRC Press, Boca Raton, FL, 1992.

Alikbangon / Commelina diffusa/climbing dayflower: Philippine Medicinal Herbs/Philippine Herbal Medicine. (accessed 2023-02-27).

MD. Shah, U.J.A. Souza, M. Iqbal, The Potential Protective Effect of Commelina Nudiflora L. against Carbon Tetrachloride (CCl4)-Induced Hepatotoxicity in Rats, Mediated by Suppression of Oxidative Stress and Inflammation, Environ. Health Prev. Med., 2017, 22 (1), 1-19.

R.A. Suganya, G.J. Jothi Preliminary Phytochemical Screening, Antibacterial and Antioxidant Activities of Commelina Nudiflora (Commelinaceae). Int. Res. J. Pharm. 2014, 5 (11), 851–855.

F.G. Mann, B.C. Saunders, Practical Organic Chemistry, London, Longmans, 1960.

J. Shinoda, Color reaction of flavone and flavonol derivatives and the like, J. pharm. Soc. Japan, 1928, 48, 214-220.

R.Y.M. Agarwal, Phytochemical Analysis of Some Medicinal Plants, Liaquat Med. Res. J., 2021, 3, 1–5.

O. Otshudi, A. Longanga, A. Vercruysse, A. Foriers, Contribution to the Ethnobotanical, Phytochemical and Pharmacological Studies of Traditionally Used Medicinal Plants in the Treatment of Dysentery and Diarrhoea in Lomela Area, Democratic Republic of Congo (DRC),

J. Ethnopharmacol., 2000, 71, 411–423.

JB Harborne, T.J. Marby, The Flavonoids, Advances in Research, Chapman and Hall, London, New York, 1982.

S.B. Mishra, V.K. Mohan, R.B. Arora, Some observations on hypoglycaemic activity of Momordica charantia, Indian Journal of Medical Research, 1960, 48, 471-477.

E. Lederer, M. Ledrer, Chromatography, Elsevier Publishing Company, New York, 1957.

E.L. Hirst, J.K.N. Jones, J. Chem. Soc., 1999, 127, 628.

S.I.A. Hakomori, Rapid Permethylation of Glycolipid, and Polysaccharide Catalyzed by Methylsulfinyl Carbanion in Dimethyl Sulfoxide, J. Biochem., 1964, 55, 205–208.

J.B. Horborne, Plant polyphenols—XIV, Phytochemistry, 1965, 4, 107-120.

A. Chakravarty, In Vitro Anti-Inflammatory Activity of a New Allelochemical from the Bark of Pithecellobium Dulce (Roxb.) Benth. R, Indian J. Chem., 2017, 56B, 447-452.

NPJ Price, Permethylation Linkage Analysis Techniques for Residual Carbohydrates, Appl. Biochem. Biotechnol., 2008, 148 (1–3), 271–276.

M.A. Hossain, M.S. Rahman, Isolation and Characterisation of Flavonoids from the Leaves of Medicinal Plant Orthosiphon Stamineus, Arab. J. Chem., 2015, 8, 218–221.

E. Bombardelli, A. Bonati, B. Gabetta, G. Mustich, Fitoterapia, 1972, 35.

P. Chatterjee, S. Chandra, P. Dey, S. Bhattacharya, Evaluation of Anti-Inflammatory Effects of Green Tea and Black Tea: A Comparative in Vitro Study, J. Adv. Pharm. Technol. Res., 2012, 3, 136-138.

S. Chandra, P. Chatterjee, P. Dey, S. Bhattacharya, Evaluation of in Vitro Anti-Inflammatory Activity of Coffee against the Denaturation of Protein, Asian Pac. J. Trop. Biomed., 2012, 2, S178–S180.



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