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Cytotoxic effects of three different oral antiseptic solutions on epithelial cells of buccal mucosa

Tatjana Kanjevac, Miona Sekulic, Mirjana Radovic, Aleksandra Vlaskovic, Aleksandar Acovic, Dejan Zdravkovic, Zoran Milosavljevic


There is a great number of different oral antiseptic solutions on the market. The effects of antiseptic solutions reflect in number of bacteria reduction, antiplaque effect, anti-inflammatory and anti-cariogenic effects and also in eliminating halitosis. In numerous studies that tested antimicrobial effect of different antiseptic solutions, it was found that all solutions have certain antimicrobial effect that is particularly expressed in solutions with octenidine and chlorhexidine. There are few data on cytotoxicity of antiseptics on keratinocytes of buccal mucosa. Aim of this study was to test and compare cytotoxic effects of three different commercial oral antiseptic solutions on keratinocytes from smear of buccal mucosa. Pilot experimental study. Solutions used in the study are: Hibidex DAP* 0.12%, Ozоsept* and Octenisept* sprays. Smears were taken from buccal mucosa of six healthy volunteers. The study was designed by using 90% antiseptic solution with cells. Cytotoxic effect of antiseptic solution on cells was measured after one, three, five, ten, fifteen, twenty, twenty five and thirty minutes by preparing sample with cells that were in antiseptic solution. The samples were stained with acridine orange/ethidium bromide (AO/EB), and observed under a fluorescence microscope. The cytotoxic effect was expressed in percentage of live cells. Testing cytotoxicity of oral antiseptic solutions Hibidex DAP*, Octenisept* and Ozosept* has showed that the viability of keratinocytes taken from the buccal mucous membranes which are under the influence of the solutions, decreases progressively in time. We noticed that the cytotoxic effects of some solutions manifested gradually, while the transitional forms of viability had not been verified when it came to the effect of Hibidex DAP*, because in a very short period of time it showed 100% negative effect on cell viability. Octenisept* and Ozosept* compared to Hibidex DAP*, have a lower level of cytotoxicity of the active ingredient for oral mucosal keratinocytes.

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