The determination of the folic acid and its selected derivatives
Abstract
Folic acid (FA) belongs to vitamins from group B. The most commonly used drug from a group of structural analogues of FA is methotrexate (MTX), showing antiproliferatative properties. Unfortunately, the action of MTX is not selective. Toxic effects of methotrexate may be partially abolished by the use of leucovorin (calcium salt of folinic acid, FNA) in conserving therapy. The aim of the presented paper was to develop a new analytical procedure using the capillary electrophoresis, allowing the separation and the quantitative marking of the folic acid, methotrexate and calcium salt of folinic acid. Optimal conditions for the electrophoretic separation were obtained for the following parameters: buffer - 25 mmol/L Na2B4O7,pH 9,76; voltage -30 kV, measurement temperature -30 °C, the introduction of a sample to the capillary - hydrodynamically 6 s (0,5 psi). These parameters allowed to obtain LOD 0,6 μM for FA, 0,65 μM for FNA and 0,7 μM for MTX.
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DOI: http://dx.doi.org/10.13171/mjc.3.4.2014.09.24.12.29
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